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目的 探讨人类免疫缺陷病毒(human immunodeficiency virus,HIV)感染者接受抗逆转录病毒治疗(antiretroviral therapy,ART)过程中α4β7high CD4~+T细胞的变化特征,并揭示该细胞亚群在免疫恢复中的潜在意义。方法 选取2012—2018年在解放军总医院第五医学中心确诊并接受ART满5年的28例HIV感染者作为研究对象。分别于基线(治疗前)及治疗后1、3、5年采集外周静脉血样本,采用密度梯度离心法分离外周血单个核细胞(peripheral blood mononuclear cells,PBMCs)。运用流式细胞技术检测CD4~+T细胞绝对计数,α4β7high CD4~+T细胞绝对计数及CD4/CD8比值;同时采用荧光定量PCR技术定量检测PBMCs中HIV DNA水平。使用GraphPad Prism 8.0软件进行数据分析,组间比较采用配对t检验,相关性分析采用Pearson相关性检验。结果 在ART 0～3年期间,α4β7high CD4~+T细胞绝对计数呈现显著上升趋势(P<0.05),而在ART 3～5年保持相对稳定(P>0.05)。相关性分析显示,ART过程中α4β7high CD4~+T细胞绝对计数的动态变化与CD4~+T细胞绝对计数(r=0.890,P<0.0001)及CD4/CD8比值(r=0.408,P<0.0001)呈显著正相关,与HIV DNA水平呈负相关(r=-0.285,P=0.021)。结论 ART治疗期间患者α4β7high CD4~+T细胞的恢复可能与免疫重建密切相关。
Abstract：Objective This study explores the characteristics of changes in α4β7high CD4~+T cells in human immunodeficiency virus(HIV) infected individuals during antiretroviral therapy(ART) and reveals the potential significance of this cell subset in immune recovery.Methods Twenty-eight HIV-infected patients who were diagnosed at the Fifth Medical Center of the PLA General Hospital and received ART for at least five years from 2012 to 2018 were selected as the research subjects.Peripheral venous blood samples were collected at baseline(before treatment) and at 1,3,and 5 years after treatment.Peripheral blood mononuclear cells(PBMCs) were isolated from the samples by density gradient centrifugation.The absolute count of CD4~+T cells,the absolute count of α4β7high CD4~+T cells and the CD4/CD8 ratio were detected by flow cytometry.At the same time,the level of HIV DNA in PBMCs was quantitatively determined by fluorescence quantitative PCR technology.Data analysis was performed using GraphPad Prism 8.0 software.Paired t-tests were used for comparisons between groups,and Pearson correlation tests were employed for correlation analysis.Results Data analysis was performed using GraphPad Prism8.0 software.Paired t-tests were used for comparisons between groups.During the ART 0-3 year period,the count of α4β7highCD4~+T cells showed a significant upward trend(P<0.05),while it remained relatively stable during the ART 3-5 year period(P>0.05).The correlation analysis revealed that the dynamic changes in the absolute count of α4β7high CD4~+T cells during the ART process were significantly positively correlated with the CD4~+T cell count(r=0.890,P<0.0001) and the CD4/CD8 ratio(r=0.408,P<0.0001),and negatively correlated with the HIV DNA level(r=-0.285,P=0.021).Conclusion During ART,the changes of α4β7high CD4~+T cell counts may play an important role in the immune recovery in HIV infected patients.
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基本信息:
中图分类号:R512.91
引用信息:
[1]孜来古丽·米吉提,潘珂君,马海梅,等.抗逆转录病毒治疗过程中α4β7~(high) CD4~+T细胞的动态变化及其与免疫恢复的关系[J].传染病信息,2025,38(06):528-533.
基金信息:
新疆维吾尔自治区自然科学基金项目-面上项目（2024D01C134）
2025-12-25
2025-12-25