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目的 了解首都医科大学北京朝阳医院Xpert平台检测结核分枝杆菌（Mycobacterium tuberculosis, MTB）/利福平（Rifampicin, RIF）(Xpert MTB/RIF)应用情况。比较Xpert MTB/RIF检测与抗酸染色的阳性率和一致性。方法 回顾性收集首都医科大学附属北京朝阳医院自2016—2023年的临床Xpert MTB/RIF筛查结果，统计Xpert MTB/RIF检测阳性标本的科室来源、类型分布及rpoB基因的突变特点。同时对Xpert MTB/RIF检测与抗酸染色法进行阳性率进行比较。结果2016—2023年首都医科大学附属北京朝阳医院Xpert MTB/RIF检测阳性率为3.56%，大体呈逐年下降的趋势；阳性标本主要来自于呼吸科病区，多为下呼吸道标本；利福平耐药基因检出率是6.35%，以探针E突变为主。Xpert MTB/RIF检出阳性率均高于抗酸染色法（P均<0.05）,2种方法有较强的一致性，k=0.973。结论 与抗酸染色相比，Xpert MTB/RIF检测的阳性率大幅提升，对于临床诊断结核病具有更好的辅助诊断作用。
Abstract：Objective To investigate the application profile of the Xpert MTB/RIF at Beijing Chao-Yang Hospital, Capital Medical University, and to compare the positivity rates and concordance between Xpert MTB/RIF and acid-fast staining. Methods A retrospective analysis was conducted on clinical Xpert MTB/RIF results at Beijing Chao-Yang Hospital from 2016 to 2023. Data on departmental sources, specimen type distribution, and rpoB gene mutation characteristics of positive samples were collected.Positivity rates between Xpert MTB/RIF and acid-fast staining were compared. Results The positive rate of Xpert MTB/RIF testing at Beijing Chaoyang Hospital Affiliated to Capital Medical University from 2016 to 2023 was 3.56%, showing an overall declining trend year by year. Most positive specimens came from respiratory medicine wards, primarily lower respiratory tract samples. The detection rate of rifampicin-resistant genes was 6.35%, with Probe E mutations being the predominant type. The positivity rate of Xpert MTB/RIF was significantly higher than that of acid-fast staining(P <0.05), with substantial agreement between the two methods(k=0.973). Conclusion Compared to acid-fast staining, Xpert MTB/RIF significantly improves the detection rate of MTB and offers superior complementary diagnostic value for the clinical diagnosis of tuberculosis.
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基本信息:
DOI：
中图分类号:R52;R446.5
引用信息:
[1]刘振嘉,范欣,陈曦等.某综合医院Xpert平台检测结核分枝杆菌/利福平对结核病诊断的回顾性分析[J].传染病信息,2025,38(03):221-224.
基金信息: